International Journal of Pharmaceutical Chemistry and Toxicology
 
Year Of Started:      2015 Published By:   SBT JOURNALS
Frequency:   Quarterly ISSN:                APPLIED

DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR THE ESTIMATION OF TRIAMCINOLONE IN BULK AND IN TABLET FORMULATION

VIVEKKUMAR K. REDASANI*, PRITESH S. TAMBOLI,

Triamcinolone is a long acting synthetic corticosteroid with processes anti-inflammatory activity. HPTLC promotes for higher separation, efficiency, and short analysis time, low amount of mobile phase and efficient data acquisition and processing. Chromatographic separation was performed on silica gel 60F254 plates. The mobile phase used in the study comprised of chloroform: 1,4,Dioxan (7.6:2.4% v/v). The densitometric evaluation of separated zones was carried out at 238 nm. The drug was resolved with Rf values of 0.45 0.3. The accuracy and reliability of the method was assessed by evaluation of linearity in range of 400 2400 ng/spot, the proposed method was validated for linearity, accuracy, precision, specificity and robustness in accordance with International Conference on Harmonization [ICH] guidelines. The method is accurate, specific and reliable, and can be successfully used for the quality control on bulk product and pharmaceutical tablet dosage form.

 
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